Nonidet® P-40 Substitute (3 Citations)

Nonidet® P-40 Substitute

Nonidet® P-40 Substitute is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.

Proteomic Grade: DG001, DG002, DG501, DG514

Research Grade: 786-511, 786-512

Proteomic Grade Detergent Solutions

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.   

G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered  in a crimp style vial as sterile, 10% aqueous solutions, sealed under inert gas and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.   

Other Proteomic Grade Detergent Solutions offered include: Triton® X-100, Triton® X-114, Brij® 35, Brij® 58, Tween® 20 and Tween® 80. A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic grade detergents, as well as one gram samples of one of our Zwitterionic detergents (CHAPS) and one of our non-detergent sulfobetaines (NDSB 201).

Features

• Full Name: Nonylphenyl-polyethylene glycol
• Molecular Formula: C₁₅H₂₄O[C₂H₄O]_n
• Molecular Weight: 573 (for n=8)
• Absorbance (254nm): 0.14 (0.05% w/v)
• Critical Micelle Concentration (CMC): ~0.05-0.3mM (25ºC)
• Cloud Point: 76ºC

Applications

• Non-ionic detergent for solubilizing membrane proteins during isolation of membrane-protein complexes
• Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds