EDC

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.

EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.

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Features

• EDC mediated coupling efficiency improved by N-hydroxysuccinimide
• Allows for 2-step coupling process
• Water soluble
• CAS Number: 25952-53-8
• Molecular Formula: C₈H₁₇N₃•HCl
• Molecular Weight: 191.71 g/mol
• Spacer Arm (Å): 0
• Reactive Group: Carbodiimide
• Reactive Toward: Primary Amine + Carboxyl
• Membrane Permeable: NO
• Water Soluble: YES
• Cleavable/Reversible: NO
• Reaction pH: 4.7 to 6.0

Applications

• Couple haptens or peptides to carrier proteins
• Conjugate carboxyl to amine groups in peptides and proteins 
• Cross link proteins to carboxyl surfaces and beads
• Convert carboxyls to amine-reactive Sulfo-NHS esters
• Activate nanoparticles to amine-reactive Sulfo-NHS esters
• DNA Labeling through 5' phosphate groups