Hydroxylamine·HCl

Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups (i.e. EGS) and for mutagenesis of plasmid DNA.

Features

• Synonym: Hydroxylammonium chloride
• Linear formula: NH₂OH•HCl
• CAS #. 5470-11-1
• Molecular weight: 69.49
• Form: White crystalline powder

Applications

• Expose protected sulfhydryl groups on SAT-modified molecules
• Cleave carbonyl containing crosslinkers, such as EGS (Ref. 1)
• Random mutagenesis of plasmid DNA for plasmid shuffle or screening for ts mutants (Ref. 2)