picoLUCENT™ PLUS-HRP

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. 

picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. 

picoLUCENT™ PLUS-HRP reagents are available in five sizes suitable for 5, 25, 50, 125 and 250 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm)

In addition, picoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP-BLOCKER™) and wash buffer (femto-TBST™).  The picoLUCENT™ PLUS-HRP kits allow detection of low picogram levels (10^-12) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm² of PVDF or nitrocellulose membrane.  

For a complete chemiluminescence immunodetection system, we offer optional horesradish perxodase (HRP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

Features

• Low picogram detection (10^-12)
• Low Background : High signal ratio
• 1 year shelf life
• Reagent for 25, 50 and 125 mini blots
• Nitrocellulose or PVDF compatible
• Kit formats supplied with optional HRP or AP conjugated secondary antibodies.