femtoLUCENT™ PLUS-HRP

femtoLUCENT™ PLUS is a femtogram level sensitive chemiluminescence immunodetection system that allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1, 2 dioxetane, a supersensitive detection reagent for HRP (horseradish peroxidase).  The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. 

femtoLUCENT™ PLUS-HRP reagents are available in three sizes suitable for 5, 25, 50 and 125 blots as each 4ml of working solution is suitable for 1 mini blot (8 x 7.5cm)

In addition, femtoLUCENT™ PLUS-HRP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and  wash buffer (femto-TBST™).  The femtoLUCENT™ PLUS-HRP kits allow detection of low femtogram levels (10^-15) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm² of PVDF or nitrocellulose membrane.

For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

Features

• Reagents alone or kit formats
• Intense light emission with low background and high signal
• Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background
• Economical: greater value compared to similar products

Applications

• Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes)
• Immunodetection of proteins and antigens for Western blots and dot blot applications
• Low femtogram detection (10^-15)that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot