The fixing of proteins in polyacrylamide gels prevents the diffusion of the proteins, thus keeping protein bands sharp and correctly resolved, and washes away running buffer components that may interfere with other downstream applications, such as staining. A major interfering agent is the SDS detergent used in SDS PAGE.
Protein fixation in polyacrylamide gels involves the denaturation and precipitation of the proteins into large insoluble aggregates within the gel matrix.
G-Biosciences SDS-PAGE Gel Fixing Solution is a relatively fast and convenient solution for fixing proteins in polyacrylamide gels and remove the SDS to prevent downstream interference.
Features
- Fix proteins in protein gel
- Wash away interfering agents (i.e. SDS)
- Convenient sizes (0.5L, 1L and 1 gal cubitainer)
Applications
- Fix proteins in polyacrylamide gels for further downstream applications
