| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-1892 | Immobilized AMP | 2 ml equivalent, dry form | $307.00 |
AMP is immobilized on agarose using the primary amine on the purine ring via a 6-carbon space arm.
Immobilized AMP is an affinity resin used in purifying NAD+-dehydrogenases such as aldehyde dehydrogenase, NADH oxidase and Lactate Dehydrogenase Isoenzyme-5, and ATP-dependent enzymes such as DNA polymerase.
Click here to request bulk or custom sizes.
Features:
Application(s):
Immobilized AMP is used for purifying NAD+-dehydrogenases and ATP dependent enzymes
Affinity Resin Preparation
Attachment of affinity ligands to immobilized supports can be accomplished using a wide variety of coupling chemistries, different linkers, and various ligand orientations to attach the ligand to the support. The linkers, ligand orientation, and coupling chemistry used can have widely varying effects on the affinity ligand's ability to bind to the desired target molecule during purification. The binding of a particular target using conditions established with another, similar resin may not always correlate directly. As such, optimization of the purification conditions may be required by the end user to achieve the desired results.
We have seen from previous studies that the linker molecule and ligand orientation used can have a significant influence on the purification results. GBio can manufacture affinity resins using any combination of coupling chemistry and linker to provide custom solutions for your research. Please inquire about our custom synthesis options.
AMP is immobilized on agarose using the primary amine on the purine ring via a 6-carbon space arm.
Immobilized AMP is an affinity resin used in purifying NAD+-dehydrogenases such as aldehyde dehydrogenase, NADH oxidase and Lactate Dehydrogenase Isoenzyme-5, and ATP-dependent enzymes such as DNA polymerase.
Click here to request bulk or custom sizes.
Features:
Application(s):
Immobilized AMP is used for purifying NAD+-dehydrogenases and ATP dependent enzymes
Affinity Resin Preparation
Attachment of affinity ligands to immobilized supports can be accomplished using a wide variety of coupling chemistries, different linkers, and various ligand orientations to attach the ligand to the support. The linkers, ligand orientation, and coupling chemistry used can have widely varying effects on the affinity ligand's ability to bind to the desired target molecule during purification. The binding of a particular target using conditions established with another, similar resin may not always correlate directly. As such, optimization of the purification conditions may be required by the end user to achieve the desired results.
We have seen from previous studies that the linker molecule and ligand orientation used can have a significant influence on the purification results. GBio can manufacture affinity resins using any combination of coupling chemistry and linker to provide custom solutions for your research. Please inquire about our custom synthesis options.
