Protein G Magnetic Beads

G-Biosciences' Protein G Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences magnetic beads compare well to leading magnetic beads (Figures 2 & 3).

 

The use of magnetic beads offers several distinct advantages of traditional immunoprecipitation experiments include a significant reduction in time and non-specific protein binding.

 

A simple protocol involves the addition of your antibody of choice to the beads, which bind the Fc region (See Table 1) during a short incubation. The tube is placed on a magnet and the supernatant is removed by aspiration. The antibody bound magnetic beads can be used in a variety of downstream processes including:

  • Immunoprecipitations
  • Co-immunoprecipitations
  • Chromatin immunoprecipitation (ChIP)
  • Small-scale IgG Purification and antibody labeling
  • Protein Isolation

 


Features

  • 1µm beads
  • 260µg human IgG/ml
  • Significant reduction in background due to non-specific binding
  • Simple, short procedure
  • No costly columns required
  • No time consuming centrifugations required
  • Gentle separation preserves native protein interactions

 


Applications

  • Immunoprecipitations
  • Co-immunoprecipitations
  • Chromatin immunoprecipitation (ChIP)
  • Small-scale IgG Purification and antibody labeling
  • Protein Isolation

 

 

Write a review

    Bad           Good

Protocol
786-904
786-905
Material Safety Data Sheet
786-904
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
786-905
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Technical Literature
Protein A, Protein G, Protein A/G and Protein L Binding Affinity A table highlighting the binding affinities of protein A, protein G, protein A/G and Protein L.  Designed to help in the choice of Well-Coated™ plates.


Catalog Description Size Price(USD) Qty
786-904
Protein G Magnetic Beads 1ml resin
$159.00
786-905
Protein G Magnetic Beads 5ml resin
$633.00