PAGE-Perfect™ (5 Citations)
Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyze and lack reproducibility.
PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels. Treat (1-100µl) protein solution with Universal Protein Precipitation Agent (UPPA™), which results in quantitative precipitation of the protein solution. Protein precipitation is not affected by the presence of detergents, chaotropes, or other common laboratory agents. The protein precipitate is collected by centrifugation and washed to remove any interfering agents such as detergents, salts, lipids, and other laboratory agents. Suspend the precipitate in the sample-loading buffer for loading on the gel for electrophoresis.
PAGE-Perfect™ effects the clean-up of 10µg mouse liver lysate that contain the following contaminants:
0.1M Tris.HCl (pH7.5) (Control) Triton® X-100 (10%)
Sodium chloride (1M) Urea (8M)
Ammonium sulfate (2.5M) CHAPS (10%)
SDS (20%) Sucrose (40%)
Removes electrophoresis interfering agents, including:
Concentrates and cleans dilute (>1ng/ml) protein samples.
Increase gel quality and reproducibility.
Protein recovery >99%.
Process 50 x 1-100µl protein samples.
- Suitable for cleaning and concentrating protein solutions for electrophoresis and other applications
|Material Safety Data Sheet|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Removal of Interfering Agents Improves Protein Electrophoresis||Removal of Detergents, Salts, Chaotropes and Reducing Agents with PAGE-Perfect™|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|
|Western Blotting Handbook||From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.|