Secondary Antibodies (29 Citations)
We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a buffer containing 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.1, with 15 mg/mL Bovine Serum Albumin (BSA) and 0.01% thimerosal.
Our affinity purified horseradish peroxidase is a 44kDa glycoprotein, with 4 lysine residues, for conjugation to a labeled molecule. It produces a colored, fluorimetric, or luminescent derivative of the labeled molecule, allowing it to be detected and quantified. HRP is ideal for secondary antibody conjugation because it is smaller, more stable, and less expensive than other popular alternatives. It also has a high turnover rate that allows the generation of strong signals in a relatively short time span. The activity of the HRP enzyme is inhibited by cyanides, azides, and sulfides.
Our affinity purified alkaline phosphatase is a large 140kDa protein that hydrolyzes phosphate groups from substrates, resulting in a colored, fluorimetric, or luminescent derivative.
- Alkaline Phosphatase (786-R43, 786-R44, 786-R45, 786-R46, 786-R47, 786-R49)
- Horseradish Peroxidase (786-R38, 786-R39, 786-R40, 786-R41, 786-R42, 786-R48)
- Goat (786-R38, 786-R39, 786-R40, 786-R41, 786-R43, 786-R44, 786-R45, 786-R46)
- Rabbit (786-R42, 786-R47, 786-R48, 786-R49)
- Mouse (786-R38, 786-R43)
- Goat (786-R42, 786-R47)
- Rat (786-R40, 786-R45)
- Rabbit (786-R39, 786-R44)
- Human (786-R41, 786-R46, 786-R48, 786-R49)
- For antibody conjugation to labeled molecules
- Recommended Working Dilutions:
|ELISA and Western blots||1:5,000 to 1:100,000|
|Immunohistochemistry||1:500 to 1:5,000|
|Material Safety Data Sheet|
|Assay Development||A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.|
|Western Blotting Handbook||From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.|