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Secondary Antibodies (29 Citations)

We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a buffer containing 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.1, with 15 mg/mL Bovine Serum Albumin (BSA) and 0.01% thimerosal.
 
Our affinity purified horseradish peroxidase is a 44kDa glycoprotein, with 4 lysine residues, for conjugation to a labeled molecule. It produces a colored, fluorimetric, or luminescent derivative of the labeled molecule, allowing it to be detected and quantified. HRP is ideal for secondary antibody conjugation because it is smaller, more stable, and less expensive than other popular alternatives. It also has a high turnover rate that allows the generation of strong signals in a relatively short time span. The activity of the HRP enzyme is inhibited by cyanides, azides, and sulfides. 
 
Our affinity purified alkaline phosphatase is a large 140kDa protein that hydrolyzes phosphate groups from substrates, resulting in a colored, fluorimetric, or luminescent derivative.

Type: Secondary

Antigen: IgG

Conjugates:

  • Alkaline Phosphatase (786-R43, 786-R44, 786-R45, 786-R46, 786-R47, 786-R49)
  • Horseradish Peroxidase (786-R38, 786-R39, 786-R40, 786-R41, 786-R42, 786-R48)

Host:

  • Goat (786-R38, 786-R39, 786-R40, 786-R41, 786-R43, 786-R44, 786-R45, 786-R46)
  • Rabbit (786-R42, 786-R47, 786-R48, 786-R49)

Reactivity:

  • Mouse (786-R38, 786-R43)
  • Goat (786-R42, 786-R47)
  • Rat (786-R40, 786-R45)
  • Rabbit (786-R39, 786-R44)
  • Human (786-R41, 786-R46, 786-R48, 786-R49)

Specifications:

 ChIP  No
 ELISA  Yes
 Flow Cytometry  Yes
 Gel Shift  No
 Immunochemistry  Yes
 Immunofluorescence  No
 Immunoprecipitation  No
 Monoclonal/Polyclonal  Polyclonal
 Neutralization  No
 Western Blot  Yes

Applications

  • For antibody conjugation to labeled molecules
  • Recommended Working Dilutions:
 ELISA and Western blots  1:5,000 to 1:100,000
 Immunohistochemistry  1:500 to 1:5,000
Protocol
786-R38
786-R39
786-R40
786-R41
786-R42
786-R43
786-R44
786-R45
786-R46
786-R47
786-R48
786-R49
Material Safety Data Sheet
786-R38
English_US
Danish
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Finnish
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786-R39
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R40
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R41
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R42
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R43
English_US
Danish
Dutch
English_UK
French
German
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Finnish
Swedish
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786-R44
English_US
Danish
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English_UK
French
German
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Portuguese
Finnish
Swedish
Polish
786-R45
English_US
Danish
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English_UK
French
German
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Norwegian
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Finnish
Swedish
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786-R46
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
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Finnish
Swedish
Polish
786-R47
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R48
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-R49
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Bioassays Handbook
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.

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