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Proteinase K Assay [Colorimetric]

Proteinase K Assay [Colorimetric]
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Proteinase K Assay [Colorimetric]
Proteinase K Assay [Colorimetric]
Proteinase K Assay [Colorimetric]
Proteinase K Assay [Colorimetric]
Proteinase K Assay [Colorimetric]
Catalog Description Size Price (USD) Qty
786-1807Proteinase K Assay [Colorimetric]100 assays$216.00
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  • Description
    Proteinase K Assay [Colorimetric] is designed for rapid and reproducible quantification of Proteinase K activity. Proteinase K Assay [Colorimetric] is based on hydrolysis of denatured hemoglobin by proteinase K which releases Folin positive amino acids and peptides. The amino acids and peptide react with Folin’s reagent to give colored product which is measured at absorbance of 578nm.
     
    Proteinase K is widely used in molecular biology for isolation and purification of nucleic acids from various tissue and cell samples. The proteolytic activity of proteinase K helps in removing nucleases from the sample, there by protecting DNA and RNA from degradation.
     
    Recently, Proteinase K is widely used in COVID-19 virus diagnostic assays for processing samples such as saliva, nasopharyngeal and oropharyngeal swabs. After proteinase K treatment the samples are heat treated to inactivate proteinase K as well as inactivate any residual pathogens or viruses left in sample. Proteinase K assay can help in optimizing sample processing step for the COVID-19 diagnostic assay.
     
    Features:
    • Rapid and reproducible
    • Linear detection range: 5µg/ml- 100µg/ml
    • Available in 100 assay tube format, adaptable to microwell assay format.
     
    Applications:
    • Estimates proteinase K activity in various samples.
    • Can be used in optimizing sample processing step for the COVID-19 diagnostic assay.
  • Reviews
Proteinase K Assay [Colorimetric] is designed for rapid and reproducible quantification of Proteinase K activity. Proteinase K Assay [Colorimetric] is based on hydrolysis of denatured hemoglobin by proteinase K which releases Folin positive amino acids and peptides. The amino acids and peptide react with Folin’s reagent to give colored product which is measured at absorbance of 578nm.
 
Proteinase K is widely used in molecular biology for isolation and purification of nucleic acids from various tissue and cell samples. The proteolytic activity of proteinase K helps in removing nucleases from the sample, there by protecting DNA and RNA from degradation.
 
Recently, Proteinase K is widely used in COVID-19 virus diagnostic assays for processing samples such as saliva, nasopharyngeal and oropharyngeal swabs. After proteinase K treatment the samples are heat treated to inactivate proteinase K as well as inactivate any residual pathogens or viruses left in sample. Proteinase K assay can help in optimizing sample processing step for the COVID-19 diagnostic assay.
 
Features:
  • Rapid and reproducible
  • Linear detection range: 5µg/ml- 100µg/ml
  • Available in 100 assay tube format, adaptable to microwell assay format.
 
Applications:
  • Estimates proteinase K activity in various samples.
  • Can be used in optimizing sample processing step for the COVID-19 diagnostic assay.

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