Reverse Transcriptase

Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. This RT is an RNA-directed DNA polymerase which lacks 3´→ 5´ exonuclease activity and can produce full-length cDNA from long RNA templates.

Product Source:

• The gene encoding recombinant MMLV Reverse Transcriptase with mutated RNase H domain is expressed in E. coli.

 Product Includes:

• Reverse Transcriptase
• 10X RT Reaction Buffer

Applications:

• First strand cDNA synthesis
• Gene expression analysis

Benefits:

• Recombinant RT with greatly reduced RNase H activity.
• Active at temperatures up to 55°C.
• Highly efficient at producing full-length cDNA from low concentration of RNA.

Unit Definition:

One unit is defined as the amount of enzyme required to incorporate 1nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

10X RT reaction buffer:

 500 mM Tris-HCl

750 mM KCl

30 mM MgCl₂

100 mM DTT

pH 8.3 @ 25°C

Storage Temperature: – 20ºC

Heat Inactivation: 65°C for 20 min