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SDS (2 Citations)

Type: Anionic detergent
 
Full Name: Sodium Dodecyl Sulfate
 
Molecular Formula: C12H25NaO4S
 
Molecular Weight: 288.38
 
Form: White to off white powder
 
Purity: >99%
 
Solubility: Water
 
Critical micelle concentration (CMC): 7-10mM (25°C)
 
Aggregation number: 62
 
Cloud point: >100°C
 
Average micellar weight: 18,000
 
SDS is also available as a 10% Solution or a 20% Solution

 

 


Applications

 

 

 

  • An anionic detergent capable of almost complete disruption of cellular structures and denaturation.
  • Used for solubilization of a wide variety of proteins, including membrane proteins, for electrophoretic separation.
  • Detergent molecules tightly bind with the protein molecules masking their native charge and rendering the protein molecules the overall negative charge of the ionic detergent.

 

 

Material Safety Data Sheet
DG092
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DG093
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Removing Detergents from Protein Solutions
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.

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