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Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems in transferring high molecular weight proteins. Other minor drawbacks also exist with the Western blotting technique and these include overheating of the apparatus, shorting out of power packs due to excess current and the messy assembling of transfer sandwiches.
SWIFT™ transfer pad (Figure 1) alleviates the above issues with Western blotting (Figure 2), when incorporated in the Western blot sandwich. Each SWIFT™ transfer pad can reduce transfer time by up to 50%, while consistently producing high efficiency transfer. The SWIFT™ transfer pad technology prevents overheating and power shortages by allowing lower chemical concentrations in the transfer buffers, without affecting transfer efficiency. The SWIFT™ transfer pad technology combines the simplicity of semi-dry sandwich assembly with the improved efficiency of wet blot transfers, reducing the need for assembly in large tanks of buffer.
SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency. (Patent Pending)
SWIFT™ is compatible with any transfer system, is supplied with or without nitrocellulose or PVDF membranes, and is available in Mini (7.5 x 8.5cm) or Medi (9.5 x 15cm). SWIFT™ Mini is for 10 Western blots and the SWIFT™ Medi is for 5 Western blots.
Features
- High efficiency protein transfer
- Reduce transfer time by up to 50%
- No overheating or power shorts
- No distortion or poor high molecular weight protein transfer
Applications
- All Western blot applications
- For improved transfer of high molecular weight proteins
