Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1). The reactive aldehyde groups are used in chemical conjugation procedures or detection of carbohydrates. For proteomic research, sodium m-periodate is used for the oxidation of the carbohydrate moiety of glycoproteins and offers the advantage of modifying the sugar side chains as opposed to critical amino acids.
The resulting aldehydes can interact with primary amines to from Schiff’s bases, which in turn can be stabilized by reduction with sodium cyanoborohydride
to form covalent amide bonds. Alternatively, the aldehydes can spontaneously react with hydrazide activated molecules to form relatively stable hydrazone bonds, which again can be stabilized with sodium cyanoborohydride.
- A mild oxidizing agent that converts carbohydrates to activated active aldehydes
- Used in coupling to amines with cyanborohydride reduction
- Synonyms: Sodium m-periodate, sodium periodate
- Linear formula:NaIO4
- CAS #. 7790-28-5
- Molecular weight: 213.89
- Form: White crystalline powder
- Oxidation of glycoproteins for coupling chemistry or detection
- For the generation of active aldehydes for reaction with primary amines to form Schiff's base
- For the generation of active aldehydes to react with hydrazide activated molecules, such as HOOK Biotin-Hydrazide