SpinOUT™ Columns
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The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples.
The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml.
The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).
Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da.
Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.
Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
Five sizes are available for each MWCO and are highlighted below:
| Cat. # | Description | # Supplied | Resin Bed Volume (ml) | Sample Load Volume (ml) |
| 786-865 | SpinOUT™ GT-100, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-866 | SpinOUT™ GT-100, 1ml | 10 | 1 | 0.05-0.1 |
| 786-867 | SpinOUT™ GT-100, 3ml | 10 | 3 | 0.1-0.5 |
| 786-868 | SpinOUT™ GT-100, 5ml | 5 | 5 | 0.5-2 |
| 786-869 | SpinOUT™ GT-100, 10ml | 5 | 10 | 0.5-4 |
| 786-703 | SpinOUT™ GT-600, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-170 | SpinOUT™ GT-600, 1ml | 10 | 1 | 0.05-0.1 |
| 786-171 | SpinOUT™ GT-600, 3ml | 10 | 3 | 0.1-0.5 |
| 786-704 | SpinOUT™ GT-600, 5ml | 5 | 5 | 0.5-2 |
| 786-705 | SpinOUT™ GT-600, 10ml | 5 | 10 | 0.5-4 |
| 786-706 | SpinOUT™ GT-1200, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-172 | SpinOUT™ GT-1200, 1ml | 10 | 1 | 0.05-0.1 |
| 786-173 | SpinOUT™ GT-1200, 3ml | 10 | 3 | 0.1-0.5 |
| 786-707 | SpinOUT™ GT-1200, 5ml | 5 | 5 | 0.5-2 |
| 786-708 | SpinOUT™ GT-1200, 10ml | 5 | 10 | 0.5-4 |
Also available in 96-well spin plate formats for processing up to 96 samples.
For the desalting and clean up of PCR reactions, click here.
Features
- Offered in 3 MWCO sizes: >700Da, >6kDa and >30kDa proteins
- Available in 5 sizes for sample volumes of 5µl to 4ml
- 96-well plate formats available
- Easy-to-use, spin-format columns for rapid purification
Applications
- Protein purification and contamination removal from samples
- For the desalting and buffer exchange of protein and nucleic acid solutions
- Separate proteins from peptides
- Desalt peptides
| Material Safety Data Sheet | |
| 786-865 | |
| 786-866 | |
| 786-867 | |
| 786-897 | |
| 786-868 | |
| 786-898 | |
| 786-869 | |
| 786-703 | |
| 786-170 | |
| 786-720 | |
| 786-171 | |
| 786-721 | |
| 786-704 | |
| 786-722 | |
| 786-705 | |
| 786-989 | |
| 786-990 | |
| 786-706 | |
| 786-172 | |
| 786-173 | |
| 786-707 | |
| 786-708 | |
| 786-991 | |
| 786-992 | |
| Technical Literature | |
| Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
| Molecular Biology Handbook | A guide to our products for DNA and RNA. |
| Plant Proteomics Handbook | |
| Protein Cross-Linking Handbook | A detailed guide to protein cross-linkers and their reactions. |
| Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
| Protein Labeling & Conjugation | |
| Protein Purification Handbook | |
| Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
- Yang, Y. et al (2016) Uniquely Identifiable DNA-Embedded Silica Nanotracer for Fractured Reservoir Characterization. PROCEEDINGS, 41st Workshop on Geothermal Reservoir Engineering. Stanford University, Stanford, California
- Wang, X. et al (2015) Zoolog Sci. 32:419
- Fedosejevs, E.T et al (2014) J Biol Chem. 289: 33412
- Wickremasinghe, N. C. et al (2014) Biomacromolecules. 15:3587
- Shane, M.W. et al (2013) Plant Physiol. 161:1634
- Vitrac, H. et al (2013) PNAS 110:9338
- Singh, J. et al (2012) Gastroenterology. 143:1308
- Singh, J. et al (2009) Am.Physiol.-Gastr. L. 297:G1206
- Gibbons, A.M. et al (2009) J. Microencaps. 26:513.
- Cryan, S. et al (2006) Mol. Pharm. 3:104
- Tripodi, K. et al (2005) Plant Physiol 139:969
- Taggart, C. et al (2005) J Exp Med 202:1659
- Yin, Z. et al (2017) Activator Protein-1: redox switch controlling structure and DNA-binding. Nucleic Acids Research. https://doi.org/10.1093/nar/gkx795
| Catalog | Description | Size | Price(USD) | Qty |
786-865 |
SpinOUT™ GT-100, 0.1ml | 25 columns |
$116.00
|
|
786-866 |
SpinOUT™ GT-100, 1ml | 10 columns |
$55.00
|
|
786-867 |
SpinOUT™ GT-100, 3ml | 10 columns |
$115.00
|
|
786-897 |
SpinOUT™ GT-100, 3ml | 5 x 10/bag |
$380.00
|
|
786-868 |
SpinOUT™ GT-100, 5ml | 5 columns |
$134.00
|
|
786-898 |
SpinOUT™ GT-100, 5ml | 50 columns |
$466.00
|
|
786-869 |
SpinOUT™ GT-100, 10ml | 5 columns |
$219.00
|
|
786-703 |
SpinOUT™ GT-600, 0.1ml | 25 columns |
$116.00
|
|
786-170 |
SpinOUT™ GT-600, 1ml | 10 Columns |
$55.00
|
|
786-720 |
SpinOUT™ GT-600, 1ml | 5 x 10/bag |
$273.00
|
|
786-171 |
SpinOUT™ GT-600, 3ml | 10 Columns |
$87.00
|
|
786-721 |
SpinOUT™ GT-600, 3ml | 5 x 10/bag |
$380.00
|
|
786-704 |
SpinOUT™ GT-600, 5ml | 5 columns |
$96.00
|
|
786-722 |
SpinOUT™ GT-600, 5ml | 50 columns |
$381.00
|
|
786-705 |
SpinOUT™ GT-600, 10ml | 5 columns |
$143.00
|
|
786-989 |
SpinOut™ GT-600 Spin Plate | 2 plates |
$229.00
|
|
786-990 |
SpinOut™ GT-600 Spin Plate | 4 plates |
$413.00
|
|
786-706 |
SpinOUT™ GT-1200, 0.1ml | 25 columns |
$116.00
|
|
786-172 |
SpinOUT™ GT-1200, 1ml | 10 Columns |
$55.00
|
|
786-173 |
SpinOUT™ GT-1200, 3ml | 10 Columns |
$87.00
|
|
786-707 |
SpinOUT™ GT-1200, 5ml | 5 columns |
$81.00
|
|
786-708 |
SpinOUT™ GT-1200, 10ml | 5 columns |
$109.00
|
|
786-991 |
SpinOut™ GT-1200 Spin Plate | 2 plates |
$229.00
|
|
786-992 |
SpinOut™ GT-1200 Spin Plate | 4 plates |
$415.00
|
