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SpinOUT™ Columns (13 Citations)

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The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples.
 
The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml.
 
The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).
 
Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da.
 
Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.
 
Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
 

Five sizes are available for each MWCO and are highlighted below:

Cat. # Description # Supplied Resin Bed Volume (ml) Sample Load Volume (ml)
786-865 SpinOUT™ GT-100, 0.1ml 25 0.1 0.005-0.02
786-866 SpinOUT™ GT-100, 1ml 10 1 0.05-0.1
786-867 SpinOUT™ GT-100, 3ml 10 3 0.1-0.5
786-868 SpinOUT™ GT-100, 5ml 5 5 0.5-2
786-869 SpinOUT™ GT-100, 10ml 5 10 0.5-4
786-703 SpinOUT™ GT-600, 0.1ml 25 0.1 0.005-0.02
786-170 SpinOUT™ GT-600, 1ml 10 1 0.05-0.1
786-171 SpinOUT™ GT-600, 3ml 10 3 0.1-0.5
786-704 SpinOUT™ GT-600, 5ml 5 5 0.5-2
786-705 SpinOUT™ GT-600, 10ml 5 10 0.5-4
786-706 SpinOUT™ GT-1200, 0.1ml 25 0.1 0.005-0.02
786-172 SpinOUT™ GT-1200, 1ml 10 1 0.05-0.1
786-173 SpinOUT™ GT-1200, 3ml 10 3 0.1-0.5
786-707 SpinOUT™ GT-1200, 5ml 5 5 0.5-2
786-708 SpinOUT™ GT-1200, 10ml 5 10 0.5-4

 

Also available in 96-well spin plate formats for processing up to 96 samples. 

 

For the desalting and clean up of PCR reactions, click here.

 


Features

  • Offered in 3 MWCO sizes: >700Da,  >6kDa and >30kDa proteins 
  • Available in 5 sizes for sample volumes of 5µl to 4ml
  • 96-well plate formats available
  • Easy-to-use, spin-format columns for rapid purification

 


Applications

  • Protein purification and contamination removal from samples
  • For the desalting and buffer exchange of protein and nucleic acid solutions
  • Separate proteins from peptides
  • Desalt peptides 
Protocol
786-865
786-866
786-867
786-897
786-868
786-898
786-869
786-703
786-170
786-720
786-171
786-721
786-704
786-722
786-705
786-989
786-990
786-706
786-172
786-173
786-707
786-708
786-991
786-992
Material Safety Data Sheet
786-865
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786-866
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786-867
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786-897
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Finnish
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786-868
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786-898
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Finnish
Swedish
Polish
786-869
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Portuguese
Finnish
Swedish
Polish
786-703
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-170
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-720
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-171
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-721
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-704
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-722
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-705
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-989
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-990
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-706
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-172
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-173
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-707
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-708
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-991
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-992
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Plant Proteomics Handbook
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Protein Labeling & Conjugation
Protein Purification Handbook
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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