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The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples.
The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml.
The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da for SpinOUT™ GT-600 and <1,500Da for SpinOUT™ GT-1200).
Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da.
Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp.
Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
Five sizes are available for each MWCO and are highlighted below:
Cat. # |
Description |
# Supplied |
Resin Bed Volume (ml) |
Sample Load Volume (ml) |
786-865 |
SpinOUT™ GT-100, 0.1ml |
25 |
0.1 |
0.005-0.02 |
786-866 |
SpinOUT™ GT-100, 1ml |
10 |
1 |
0.05-0.1 |
786-867 |
SpinOUT™ GT-100, 3ml |
10 |
3 |
0.1-0.5 |
786-868 |
SpinOUT™ GT-100, 5ml |
5 |
5 |
0.5-2 |
786-869 |
SpinOUT™ GT-100, 10ml |
5 |
10 |
0.5-4 |
786-703 |
SpinOUT™ GT-600, 0.1ml |
25 |
0.1 |
0.005-0.02 |
786-170 |
SpinOUT™ GT-600, 1ml |
10 |
1 |
0.05-0.1 |
786-171 |
SpinOUT™ GT-600, 3ml |
10 |
3 |
0.1-0.5 |
786-704 |
SpinOUT™ GT-600, 5ml |
5 |
5 |
0.5-2 |
786-705 |
SpinOUT™ GT-600, 10ml |
5 |
10 |
0.5-4 |
786-706 |
SpinOUT™ GT-1200, 0.1ml |
25 |
0.1 |
0.005-0.02 |
786-172 |
SpinOUT™ GT-1200, 1ml |
10 |
1 |
0.05-0.1 |
786-173 |
SpinOUT™ GT-1200, 3ml |
10 |
3 |
0.1-0.5 |
786-707 |
SpinOUT™ GT-1200, 5ml |
5 |
5 |
0.5-2 |
786-708 |
SpinOUT™ GT-1200, 10ml |
5 |
10 |
0.5-4 |
Also available in 96-well spin plate formats for processing up to 96 samples.
For the desalting and clean up of PCR reactions, click here.
Features
- Offered in 3 MWCO sizes: >700Da, >6kDa and >30kDa proteins
- Available in 5 sizes for sample volumes of 5µl to 4ml
- 96-well plate formats available
- Easy-to-use, spin-format columns for rapid purification
Applications
- Protein purification and contamination removal from samples
- For the desalting and buffer exchange of protein and nucleic acid solutions
- Separate proteins from peptides
- Desalt peptides