T4 DNA Polymerase

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction with the presence of template and primer. It has both DNA-dependent DNA polymerase activity and 3´→5´ exonuclease activity which make the enzyme useful for several molecular biology applications such as:

• 3´-overhang removal to form blunt ends.
• 5´-overhang fill-in to form blunt ends.
• Probe labeling using replacement synthesis.
• DNA library preparation for Next-generation sequencing.
• Ligation-independent cloning of PCR products.
• Second strand synthesis in site-directed mutagenesis.

Source

E. coli cells with a cloned gene of bacteriophage T4 DNA Polymerase

Product includes        

• T4 DNA Polymerase
• 10X T4 DNA Polymerase buffer

10X T4 DNA Polymerase reaction buffer composition

500 mM Tris-HCl, 100 mM MgCl₂, 50 mM dithiothreitol, pH 7.5 @ 25°C.

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC.

Unit Definition:

One unit of T4 DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.

Molecular Weight

Theoretical: 104 kDa

Storage temperature

- 20°C.