| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2197 | Taq DNA Ligase | 2000 U | $85.00 | |
| 786-2198 | Taq DNA Ligase | 10000 U | $325.00 |
Taq DNA Ligase uses NAD+ as a cofactor to catalyze the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini. It is active at high temperatures (50°C-70°C) and used to detect a single-base substitution.
Items Included:
Applications:
Taq DNA Ligase Buffer with NAD+ [10X] Composition:
500 mM Tris-HCl, 100 mM MgCl2, 100 mM DTT, 10 mM NAD+, pH 7.5 @ 25ºC
Storage Buffer:
50 mM Tris-HCl, 50 M KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature:
-20º C
Unit Definition:
(Cohesive End Unit)
One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.
Taq DNA Ligase uses NAD+ as a cofactor to catalyze the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini. It is active at high temperatures (50°C-70°C) and used to detect a single-base substitution.
Items Included:
Applications:
Taq DNA Ligase Buffer with NAD+ [10X] Composition:
500 mM Tris-HCl, 100 mM MgCl2, 100 mM DTT, 10 mM NAD+, pH 7.5 @ 25ºC
Storage Buffer:
50 mM Tris-HCl, 50 M KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature:
-20º C
Unit Definition:
(Cohesive End Unit)
One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.
