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Thiopropyl Resin

Thiopropyl_Resin

Synonyms: Thiol Reactive Agarose Beads, Activated thiol Sepharose

Thiopropyl Resin is used in purification of thiol group containing proteins, molecules and enzymes. The thiol-containing molecules are reversibly immobilized on the resin. When the sample to be purified is passed through the resin, thiol- containing biomolecules bind to the resin to form mixed disulfides and release2-thiopyridone. The bound molecule is eluted by addition of reducing agent such as DTT or 2-mercaptoethanol.

Specifications

  • Active group: 2-pyridyl disulfide
  • Active group concentration: 18-31 µmole 2-pyridyl disulfide/ml drained gel
  • Spacer: Glutathione
  • Coupling capacity: around 10-20 mg protein/ml of drained gel
  • Matrix: 6% cross-linked Agarose
  • pH stability: 2-8 long term, 2-8 for CIP
  • Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Do not use azides.
  • Physical stability: volume variations are negligible for changes in pH or ionic strength
  • Supplied as 50% slurry in 20% ethanol, 1 mM EDTA
 
Applications
  • Purify thiol-containing proteins and peptides from non-thiol containing proteins or peptides.
  • Purify enzyme with active thiol site from denatured enzymes
  • Store and protect thiol containing 
Protocol
786-1785
786-1786

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