Thiopropyl Resin

Synonyms: Thiol Reactive Agarose Beads, Activated thiol Sepharose

Thiopropyl Resin is used in purification of thiol group containing proteins, molecules and enzymes. The thiol-containing molecules are reversibly immobilized on the resin. When the sample to be purified is passed through the resin, thiol- containing biomolecules bind to the resin to form mixed disulfides and release2-thiopyridone. The bound molecule is eluted by addition of reducing agent such as DTT or 2-mercaptoethanol.

Specifications

• Active group: 2-pyridyl disulfide
• Active group concentration: 18-31 µmole 2-pyridyl disulfide/ml drained gel
• Spacer: 3-Carbon-SH group 
• Coupling capacity: around 10-20 mg protein/ml of drained gel
• Matrix: 6% cross-linked Agarose
• pH stability: 2-8 long term, 2-8 for CIP
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Do not use azides.
• Physical stability: volume variations are negligible for changes in pH or ionic strength
• Supplied as lyophilized powder. To rehydrate, add twice the final resin volume of binding/storage buffer and allow to swell for 15 minutes

• Purify thiol-containing proteins and peptides from non-thiol containing proteins or peptides.
• Purify enzyme with active thiol site from denatured enzymes