Southern Blot Analysis

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  • Protocol
  • BE-315
  • Southern Blot Analysis
  • 1 Classroom Demonstration
  • $94.00
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  • Protocol, Manual or Handbook

Southern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis.  DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane.  The resulting membrane can be probed for specific DNA sequences using a single stranded probe that anneals or hybridizes with a specific sequence.  The hybridization probes are often labeled, which can be detected later.
The Southern Blot Analysis kit is a class demonstration kit that allows students to visualize the Southern blot technique and visualize the blotted DNA with a safe blue stain.
 
Supplied with all reagents needed for a class demonstration, including a detailed Teacher’s Guide.
FEATURES & APPLICATIONS
    • Class demonstration of Southern blot technique.
    • Demonstrate agarose electrophoresis.
    • Introduce principle of probes and hybridization.
ADDITIONAL HARDWARE
    • Agarose electrophoresis equipment