FOCUS-Fast SilverTM

MassSpec Compatible Silver Staining for Enhanced Performance

For mass spectrometry analysis complete digestion and recovery of peptides is needed. Residual silver ions used in traditional kits inhibit trypsin digestion. Glutaraldehyde, used as a sensitizer in many silver-staining methods, modifies lysine residues preventing complete digestion and recovery of the peptides.

FOCUS-FastSilverTM - a sub-nanogram sensitive silver staining kit produces the crystal clear background, high visibility, and maximal peptide recovery needed for critical analysis by mass spectrometry.

FOCUS-FastSilverTM does not use protein modifier glutaraldehyde and the kit is provided with silver-ion removing and de-staining reagent SilverOUTTM which allows complete trypsin digestion and extraction of peptides needed for mass spectrometry.

FEATURES

Full & Efficient Trypsin Digestion - Silver has been found to inhibit trypsin digestion by binding at the active site of trypsin and chymotrypsin. FOCUS-FastSilverTM is provided with SilverOUTTM reagent for removal of silver ions from protein bands in polyacrylamide gels. This allows maximal trypsin digestion. Full and Efficient Recovery of Peptides - Glutaraldehyde modifies lysine residues and cross-link proteins, reducing peptide recovery. FOCUS-FastSilverTM does not use glutaraldehyde, used in traditional silver staining protocols. FOCUS-FastSilverTM allows enhanced trypsin digestion and efficient recovery of digested peptides. Sensitivity ~ 0.3ng protein and crystal clear background for maximum sensitivity. Short protocol time- protein can be detected in less than 90 minutes.

APPLICATOINS

Mass spectrometry compatible staining of protein gels.