HOOK™ Activated Agarose (Carboxy/Sulfhydryl Reactive)

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  • 786-065

  • HOOK™ Activated Agarose (Carboxyl/ Sulfhydryl Reactive)

  • 10ml resin
  • $76.00
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  • Protocol, Manual or Handbook MSDS, Material safety data sheet

For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their carboxy or sulfhydryl groups.
 
The carboxy/sulfhydryl reactive HOOK™ Activated Agarose is 6% agarose beads that have been activated by the addition of an aminoethyl group. Proteins and peptides are coupled to the agaroses amine group via the use of common protein crosslinkers, such as EDC (Cat. # BC25) or sulfoSMCC (Cat. # BC23). EDC will couple protein/peptide carboxyl groups to the agar ose, whereas sulfoSMCC will react with the sulfhydryl residues of the proteins/peptides. For further information on the crosslinkers see the Cross Linking & Modification section.
FEATURES
    • Binding capacity: 5mg protein/ml resin.
    • 6% cross-linked agarose.
APPLICATIONS
    • Coupling of proteins and peptides to agarose beads.
    • Suitable for antibody purification.
    • Discovery of new interacting proteins.