Trypsin is a widely used protease enzyme for protein sequence analysis. Trypsin undergoes autolysis, producing trypsin fragments that interfere with sequence analysis. Genotech has developed a chemically modified trypsin that is enzymatically active and yet resistant to autolysis.

MSG-Trypsin^TM is methylated, TPCK treated and affinity purified. Modified trypsin is quality tested for mass spectrometry use. MSG-Trypsin^TM is supplied in a convenient 20g/vial.
For mass spectrometry sequence analysis, MSG-Trypsin to protein ratio of 1:100 to 1:20 is recommended.

Unlike other Trypsin preparations, our MSG-Trypsin^TM is highly stable (figure 1), maintaining its activity in severe denaturing buffers (see figure 2) as well as after a prolonged incubation at 37˚C and as a result is shipped without dry-ice and can be stored for a long period without any loss of activity. Supplied with trypsin re-suspension buffer. We offer both bovine and porcine mass spectroscopy grade trypsin.

Figure 1: MSG-Trypsin^TM Stability compared to Competitors.

Genotechs MSG-Trypsin^TM and a competitors trypsin were incubated at 37˚C overnight. SDS-PAGE, our MSG-Trypsin^TM showed no degradation, whereas up to 80% of the competitors trypsin degraded. Silver stained gel, at zero time (0) and after overnight (o/n) incubation at 37˚C

Figure 2

FEATURES

• Modified trypsin for sequence analysis and mass spectrometry applications.
• Resistant to autolysis and degradation.
• Stable in denaturing buffers.
• Stable at ambient temperature and suitable for long-term storage.
• Recommended MSG-Trypsin^TM to protein ratio, 1:100 to 1:20.
• Available as porcine or bovine.
   

APPLICATIONS

• Digestion of proteins for sequence and peptide fragment analyses.
• Suitable for sequencing and mass spectrometry applications.