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G-Biosciences - MSG-Trypsin™

MSG-TrypsinTM

Trypsin is a widely used protease enzyme for protein sequence analysis. Trypsin undergoes autolysis, producing trypsin fragments that interfere with sequence analysis. Genotech has developed a chemically modified trypsin that is enzymatically active and yet resistant to autolysis.

MSG-TrypsinTM is methylated, TPCK treated and affinity purified. Modified trypsin is quality tested for mass spectrometry use. MSG-TrypsinTM is supplied in a convenient 20g/vial.
For mass spectrometry sequence analysis, MSG-Trypsin to protein ratio of 1:100 to 1:20 is recommended.

Unlike other Trypsin preparations, our MSG-TrypsinTM is highly stable (figure 1), maintaining its activity in severe denaturing buffers (see figure 2) as well as after a prolonged incubation at 37˚C and as a result is shipped without dry-ice and can be stored for a long period without any loss of activity. Supplied with trypsin re-suspension buffer. We offer both bovine and porcine mass spectroscopy grade trypsin.

 




Figure 1: MSG-TrypsinTM Stability compared to Competitors.

Genotechs MSG-TrypsinTM and a competitors trypsin were incubated at 37˚C overnight. SDS-PAGE, our MSG-TrypsinTM showed no degradation, whereas up to 80% of the competitors trypsin degraded. Silver stained gel, at zero time (0) and after overnight (o/n) incubation at 37˚C

Figure 2


FEATURES

  • Modified trypsin for sequence analysis and mass spectrometry applications.
  • Resistant to autolysis and degradation.
  • Stable in denaturing buffers.
  • Stable at ambient temperature and suitable for long-term storage.
  • Recommended MSG-TrypsinTM to protein ratio, 1:100 to 1:20.
  • Available as porcine or bovine.
     

APPLICATIONS

  • Digestion of proteins for sequence and peptide fragment analyses.
  • Suitable for sequencing and mass spectrometry applications.

 

Catalog #DescriptionSizeShipping
Condition
Price 
786-245MSG-Trypsin™ (Porcine)/5 vials, 20µg/vial5 x 20µg/vialAmbient US $64.00
786-245BMSG-Trypsin™ (Bovine)/5 vials, 20µg/vial5 x 20µg/vialAmbient US $64.00
   
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