Nickel Chelating Resin
Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins.
This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20-40μmoles Ni2+/ml resin.
FEATURES
- For the purification of 6x His proteins
- High capacity: ~4mg/ml
- Ligand density: 20-40μmoles Ni2+ /ml resin
- Bead Structure: 6% cross-linked agarose
APPLICATIONS
- Affinity purification of proteins with a 6x His tag.