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DetergentOUT™ Tween® Removal Spin Column

Tween 20

Prepacked, spin column format, detergent removal resin columns for polysorbate or Tween® detergents or surfactants.  The resin in the spin columns has a high binding affinity for polysorbate detergents, commercially known as Tween®. The binding capacity is approx 85mg polysorbate detergent per 1ml resin. The resin does not bind to protein allowing for a >95% protein recovery. DetergentOUT™ Tween® resin binds other commonly used detergents, but with lower affinity compared to our DetergentOUT™ GBS10.

Two sizes of column are available:

Description Sample Size (µl) Resin Volume (µl)
 DetergentOUT™ Tween® Micro 30-500 800
 DetergentOUT™ Tween® Medi 500-2,000 3,000

For other detergents, we highly recommend our DetergentOUT™ GBS10 columns and resin. The DetergentOUT™ GBS10 shows greater efficiency of detergent removal and protein recovery for other detergents, including SDS, CHAPS, Triton, Nonidet and Brij

Click here to request bulk or custom sizes.

Features

  • Fast and efficient removal of polysorbate or Tween® detergents
  • Binding capacity of 85mg/ml resin
  • >95% protein recovery

 

 

Protocol
786-214
786-215
Material Safety Data Sheet
786-214
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786-215
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Highly efficient detergent removal for improved mass spectrometry analysis  Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionization when analyzed by mass spectrometry. Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss for downstream analysis by mass spectrometry and other techniques.   Remove Free Aqueous Detergents Prevent Interference of Downstream Applications Improve Mass Spec. Detection The application note shows how DetergentOUT™ GBS10 improves mass spec detection of peptides after detergent removal.   Download the free application note to find the key to improved mass spectrometry analysis!
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Protein Purification Handbook
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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