Transformation of E. coli with p-Amylase (Lab 8C)

This kit is designed to teach 8 groups of four students the principles of bacterial transformation.

The kit contains the reagents to allow students to:

• Generate E.coli comptent cells
• Transform competent cells with the p-Amylase plasmid
• Grow transformed colonies on selection plates of LB/starch/amp agar plates
• Understand antibiotic selectivity
• Visualize the new bacterial phenotype (amylase production) on the selection plates

Features

• For 8 groups of 4 students 
• Requires waterbath and incubation oven, micropipettes and tips

Applications

• HS-LS1: From Molecules to Organisms: Structures and Processes
• LS1.A: Structure and Function