Molecular and Genomic Studies
Our Bioscience Excellence™ Molecular and Genomic Studies Kits introduce students to molecular cloning techniques, including restriction digestion, ligation, bacterial transformation and protein expression. Other techniques include the polymerase chain reaction and bacterial conjugation.
Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques. The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA.
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A major factor in the advancement of the genomic field and subsequent sequencing of the human genome was the ability to amplify the amount of genes, giving researchers an unlimited supply of DNA. This technique, which has also been a major contributor to the advancement of molecular biology, i..
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Agarose electrophoresis is an important tool for isolating DNA by its molecular weight; following separation of DNA molecules, the DNA needs to be purified from the agarose for further applications. The Purification of a Gene kit teaches the recovery and purification of DNA fragments follow..
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This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to:
Set up restriction digests of the p-Amylase plasmid with two enzymes
Set up positive and negative controls for the rest..
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Southern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis. DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane.
The resulting membrane can be probed for spec..
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This kit is designed to teach 8 groups of four students the principles of bacterial transformation.
The kit contains the reagents to allow students to:
Generate E.coli comptent cells
Transform competent cells with the p-Amylase plasmid
Grow transformed colonies on selection plates of L..
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This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.
The kit contains the reagents to allow students to:
Collect p-Amylase-transformed bacteria from an overnight culture
Resuspend bacteria, lyse and isolate the p-Amylase plasmi..
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Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance.
The Bacterial Conjugation kit contains two bacteria with different drug resistance genes and all th..
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