The expression of recombinant proteins is a popular and routinely used technique in protein studies. The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels. The aggregated proteins are difficult to solubilize, due to the nature of aggregates, however we offer a selection of products for dealing with the range of issues involved with solubilizing and recovering active proteins from inclusion bodies.
Solubilization of Hydrophobic Proteins from Inclusion Bodies
We offer IBS-HP™ Buffer for the solubilization of inclusion bodies containing highly hydrophobic proteins. The IBS-HP™ Buffer contains optimized concentration of SDS, a buffering agent and urea. After solubilization of inclusion bodies, free SDS may be quenched or removed by competing with a non-ionic detergent.
The IBS-HP™ Buffer is compatible with NI™ Protein Assay (Cat. # 786-005) and, when used with PAGE-Perfect™ kit (Cat. # 786-123) for SDS-PAGE analysis.