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Extraction and Lysis Systems

Download the Protein Sample Preparation Handbook

 

We offer a wide variety of products for protein extraction and lysis.

 

Our protein extraction and lysis buffer systems (PE LB™) ensure good protein recovery, while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays, electrophoresis, folding studies, chromatographic studies and many other downstream applications. The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, mammalian cell cultures and tissues.

 

FOCUS™ Proteome Kits are for the preparation of total protein, including soluble, insoluble, membrane, cytoplasmic, nuclear, signal, phospho- and glyco- proteins. These kits are simple to use, save time, improve the quality of protein analysis and enhance the chances of discovery of novel proteins.  They are suitable for the analysis of proteins using electrophoresis and other biochemical techniques and are compatible with biological samples from tissues, cells, plants, yeast, bacteria, insects and biological samples.

 

Our lysis buffers and kits include RIPA lysis buffer, a complete lysis buffer; an inclusion bodies solubilzation buffer, IBS™ Buffer; kits for total protein extraction (TPE™) for the extraction of total proteins from cells and tissues, and an RBC lysis buffer specifically designed for the lysis of red blood cells.

 

Accessories are available to assist with protein extraction and isolation procedures which include our easy grinding resin, a highly efficient grinding resin with matching pestles and Molecular Grinding Resin™ which is available with matching pestles and tubes. We also offer ProteaseArrest™, a general and complete protease inhibitor cocktail solution.

Model: IBS-HP™ Buffer
The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
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A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Ur..
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Bacterial PE LB™ in Phosphate buffer is a variation of Bacterial PE LB™ buffers only in phosphate buffer. It is used preferably for extraction of soluble proteins and soluble recombinant proteins from bacterial cells.   Bacterial PE LB™ in Phosphate buffer contains a mil..
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Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during..
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A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extraction of both proteins and DNA.   The resin is a neutral abrasive material that does not b..
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Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli, including membrane as well as soluble proteins.  Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme e..
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Chaotropic extraction buffers that preserve the native charge of proteins making them suitable for sample extraction for 2D gels   One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must solubil..
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Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins.     Features Single step extracti..
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Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins.  Suitable for biological samples from animal tissues and adherent and suspension c..
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Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as sol..
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Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins.  Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™..
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The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
$0.00
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