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Lysis and Extraction Systems

An array of reagents and accessories for extraction of proteins from biological samples. Depending on applications and biological sample type, researchers are offered the options to choose from one or more of the following lysis and protein extraction systems. These protein extraction systems also offer researcher the option to select buffering agents either amine based, non-amine or phosphate buffering agents. These lysis systems are designed to cover the most extensive range of applications in protein research.

The Protein Extraction and Lysis Buffer systems (PE LB™ series) utilize a mild detergent, ensuring efficient protein recovery while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays, electrophoresis, ELISA, Western blot, folding studies, chromatographic studies, and many other downstream applications. The PE LB™ systems offer a wide selection of lysis buffers for extraction of proteins from bacteria, insects, mammalian cell cultures, mammalian tissues, and yeast.

PopLysis protein extraction systems utilize a cocktail of membrane solubilizing detergents and are optimized to rapidly solubilize and “pop open” membrane structures, allowing cellular proteins to spill out into lysis solution. The cellular proteins are stable in PopLysisTM buffer and suitable for downstream applications such as enzyme assays, chromatography studies, ELISA, Western blot, protein folding studies, and gel electrophoresis. These systems are also suitable for high throughput applications such as screening recombinant proteins. PopLysisTM buffers contain amine-free buffering agents making them suitable for applications that require proteins in amine free buffers. PopLysisTM buffer systems are optimized kits for lysis and extraction of proteins from bacteria, yeast, neurons, mammalian cellsmammalian tissue, and insect cells.

FOCUS™ Proteome Kits are optimized for the preparation of total protein for downstream screening and protein discovery.  These kits have been optimized for the isolation of several protein types including soluble, insoluble, membrane, cytoplasmic, nuclear, signal, phosphoproteins and glycoproteins. These kits are simple to use, save time, improve the quality of protein analysis and improve the likelihood of novel protein discovery. FOCUS™ Proteome Kits are suitable for the analysis of proteins using 1D and 2D electrophoresis, mass-spectrometry, and other sensitive biochemical techniques.  Each kit is specifically designed for protein isolation from either mammalian cells, mammalian tissues, bacteria, insects, plants, yeast, and other biological samples.

Other lysis buffer offerings include the extensively cited RIPA lysis buffer, and a modified mild version Mild-RIPA lysis and extraction buffer that does not contain SDS detergent. Inclusion bodies solubilization buffers, IBS™ and HP- IBS™ Buffers. A kit for Total Protein Extraction (TPE™) for the extraction of total proteins from cells and tissues for electrophoresis and other applications. RBC lysis buffer specifically designed for lysis of red blood cells.

Accessories are also offered to assist with protein extraction and isolation procedures which include our EZ Grind™ and Molecular Grinding Resin™, sample grinding tools, they are available either as single use tubes with matching pestles or just grinding resin with or without matching tubes and pestles. We also offer ProteaseArrest™, a complete range of protease Inhibitor cocktail solutions. PhosphataseArrest, for phosphatase sensitive protein extraction applications. Metal chelators (EDTA & EGTA), reducing agents, various salts (both monovalent and divalent), lytic enzymes, denaturing agents, proteomic detergents, and other additives for protein research.

Download the Protein Sample Preparation Handbook

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Ure..
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Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during..
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Bacterial PE LB™ in Phosphate buffer is a variation of Bacterial PE LB™ buffers only in phosphate buffer. It is used preferably for extraction of soluble proteins and soluble recombinant proteins from bacterial cells.   Bacterial PE LB™ in Phosphate buffer contains a mild no..
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A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extraction of both proteins and DNA.   The resin is a neutral abrasive material that does not b..
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Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli, including membrane as well as soluble proteins.  Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme e..
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Chaotropic extraction buffers that preserve the native charge of proteins making them suitable for sample extraction for 2D gels   One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must solubilize ..
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Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins.     Features Single step extraction protoco..
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Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins.  Suitable for biological samples from animal tissues and adherent and suspension c..
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Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as sol..
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Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins.  Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™..
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The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
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The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggre..
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