Insect PE LB™
Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Insect Cell-PE LB™. Insect Cell-PE LB™ can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of protein from insect cells.
Insect Cell-PE LB™ is compatible with most applications, including enzyme assays, various chromatography procedures, electrophoresis, etc. Insect Cell-PE LB™ is also compatible for protein estimation with NI™ protein assay (Non-Interfering Protein Assay™). The protein extract prepared with Insect Cell-PE LB™ may be used for most enzyme assays including reporter gene assays (e.g. β-galactosidase, luciferase, chloramphenicol acetyltransferase), kinases (e.g., PKC, PKA, Tyrosine Kinase), and immunoassays (e.g., ELISA, Western blots, RIA).
Provides a simple and versatile method for protein extraction from adherent or suspended Sf9 and Sf21 insect cells
Compatible with electrophoresis and chromatographic applications
For extraction of soluble proteins from cultured insect cells
Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis
|Material Safety Data Sheet|
|Isolate Biologically Active Proteins||Our range of Protein Extraction & Lysis Buffer (PE LB™) systems isolate biologically active proteins from bacteria, yeast, mammalian cells and tissue and insect cells.|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|
|Western Blotting Handbook||From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.|