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FOCUS™ Cytoplasmic and Nuclear Proteins

Catalog Description Size Price(USD) Qty
786-248
FOCUS™ Cytoplasmic & Nuclear Proteins 50 Preps
$369.00

FOCUS™ Cytoplasmic and Nuclear Proteins

The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible with 2D gel electrophoresis
.

The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is based on the Nuclear & Cytoplasmic Extraction kit; however, it has been modified to be fully compatible with 2D electrophoresis and subsequent downstream processes.
 

The kit is provided with reagents necessary for fractionation of cytoplasmic and nuclear proteins as well as solubilization buffer suitable for IEF/2D gels for better spot resolution.  The kit is designed for >50 preps, depending on the tissue used, where one prep is 20x106 mammalian cells or 100mg mammalian tissue.

 


Features

  • Includes chaotropic extraction buffer for solubilization of nuclear and cytoplasmic proteins
  • Fully compatible with 2D analysis 

 

Applications

  • Fractionation of nuclear and cytoplasmic proteins from cells and tissues
  • Suitable for sample preparation for 2D gel electrophoresis and other applications

 

 

Protocol
786-248
Material Safety Data Sheet
786-248
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
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