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Fractionation and Enrichment Kits

The analysis of a proteome is often inhibited by the vast amount of proteins, with large abundant proteins inhibiting the signal of lower abundance and often more interesting proteins.  Researchers overcome this problem by using fractionation, however inconsistencies in techniques and buffers often result in a lack of reproducibility.
G-Biosciences offers a wide selection of fractionation kits for processing samples from cells, tissues, bacteria, yeast, plants, and other types of samples. A selection of sample preparation accessories and supplies are also available. The following kits, accessories, and supplies are suitable for the analysis of proteins using electrophoresis and other biochemical techniques.

The FOCUS line of products allow for the fractionation of a large selection of biological samples into a multitude of different fractions and these resulting fractions are compatible with 2D electrophoresis and subsequent protein identification techniques.

Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT has been specifically developed for substantial removal of albumin from such samples.

We also offer a large variety of accessories to aid in protein fractionation and enrichment procedures.

Download the Protein Purification Handbook for a full list of related products

 
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abu..
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Concanavalin A (Con A) Agarose consists of Con A coupled to agarose by the Sodium Metaperiodate method.  Con A is a tetrameric metalloprotei..
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The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasm..
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FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membr..
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FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. ..
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FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples f..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  Speci..
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FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The k..
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Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid ..
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A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, y..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.   FOC..
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2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identificat..
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HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mamm..
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PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of ph..
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The Nuclear & Cytoplasmic Extraction Kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit gener..
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