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FOCUS™ Soluble and Insoluble

Catalog Description Size Price(USD) Qty
786-247
FOCUS™ Soluble & Insoluble Kit 50 Preps
$369.00

FOCUS™ Soluble and Insoluble

A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilize difficult proteins.

 

The FOCUS™ Soluble & Insoluble kit is supplied with a specific clean-up kit for the preparation of each fraction for isoelectric focusing and 2D electrophoresis for improved spot resolution.

 
FOCUS™ Soluble & Insoluble kit is designed for 50 preps, where 1 prep is:
  • 100mg mammalian tissue
  • 50µl wet animal cell pellet
  • 50µl wet yeast pellet
  • 50µl wet bacteria pellet
  • 250mg plant tissue

 

 


Features

  •  Generates soluble and insoluble fractions.
  •  Fractions fully compatible with 2D electrophoresis.

 


Applications

  •  Extraction of soluble and insoluble proteins from tissues, cells, plants, yeast, bacteria and other sources.
  •  Suitable for sample preparation for 2D gel electrophoresis and other applications.

 

 

Protocol
786-247
Material Safety Data Sheet
786-247
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
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