FOCUS™ Soluble and Insoluble
A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilize difficult proteins.
The FOCUS™ Soluble & Insoluble kit is supplied with a specific clean-up kit for the preparation of each fraction for isoelectric focusing and 2D electrophoresis for improved spot resolution.
100mg mammalian tissue
50µl wet animal cell pellet
50µl wet yeast pellet
50µl wet bacteria pellet
250mg plant tissue
Generates soluble and insoluble fractions.
Fractions fully compatible with 2D electrophoresis.
Extraction of soluble and insoluble proteins from tissues, cells, plants, yeast, bacteria and other sources.
Suitable for sample preparation for 2D gel electrophoresis and other applications.
|Material Safety Data Sheet|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|