FOCUS™ SubCell Kit

FOCUS^™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins. The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes. The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.

 

FOCUS^™ SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2x10⁷ mammalian cells or 100mg mammalian tissue.