menu
Your Cart

FOCUS™ SubCell Kit

Catalog Description Size Price(USD) Qty
786-260
FOCUS™ SubCell 50 Preps
$358.00

FOCUS™ SubCell Kit

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.

 

FOCUS SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins. The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes. The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.
 
FOCUS SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2x107 mammalian cells or 100mg mammalian tissue.

 


Features

  • Adaptable for animal tissue
  • Resulting fractions suitable for 2D electrophoresis and other applications
  • Isolated mitochondria are ~90% active

 


Applications

  • Fractionation of cells and tissues into mitochondrial, nuclear, cytosolic and membrane proteins
  • Suitable for proteomic and cell signalling studies
Protocol
786-260
Material Safety Data Sheet
786-260
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Enrichment of Nuclear, Mitochondrial, Cytosolic and Membrane Proteins with FOCUSâ„¢ SubCell
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
Write a review
Note: HTML is not translated!
Bad Good
Captcha

Latest Blog

Download our handbooks

Custom Services

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.