2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identification is achieving suitable resolution of proteins. The high dynamic range of a species' proteome means that the more abundant proteins mask the less abundant and often more interesting proteins.
Fractionation simplifies the protein composition and allows for improved resolution and simplified 2D maps, which in turn allows for improved analysis and interpretation and greatly increases the chances of identifying novel and less abundant proteins.
Fraction-FOCUS™ utilizes proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins (see figure 1 & 2). Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.
There is no detectable loss of material total protein during the Fraction-FOCUS™ procedure. At the end of the fractionation, cellular proteins are in one of many fractions. The entire fractionation is carried out in micro scale.
The Fraction-FOCUS™ kit is designed for 10 preparations, where one preparation is: