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Immobilized Protein A

Catalog Description Size Price(USD) Qty
786-283
Protein A Resin 5ml Resin
$283.00
786-824
Protein A Resin 25ml resin
$853.00
786-825
Protein A Resin 5 x 1ml Columns
$314.00
786-826
Protein A Resin 5 column kit
$418.00
786-827
Protein A Resin 10 x 0.2ml columns
$178.00
786-828
Protein A Resin 10 column kit
$233.00
786-996
Protein A Resin Spin Plate 1 Plate
$235.00

Immobilized Protein A

Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our immobilized protein A has a high binding capacity (>40mg/ml resin).

 

The Immobilized Protein A 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 0.2ml column is designed for 25-500µl of serum or other sample and can purify up to 6-8mg IgG, based on the 30-40mg/ml binding capacity of the resin.

 

The Immobilized Protein A 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.   Each 0.2ml column is designed for 0.5-2ml of serum or other sample and can purify up to 40mg IgG, based on the 30-40mg/ml binding capacity of the resin.

 

Available in 96-well spin plate formats for processing up to 96 samples. Other formats, including FPLC columns and magnetic beads.

 

Specific binding and elution buffers are also available.

 

Click here to request bulk or custom sizes.

 


Features

  • High binding capacity: >40mg human IgG/ml resin
  • Protein A Leaching: <10ng/mg IgG
  • 30-300cm/hr flow velocity
  • Ligand: Recombinant Staphylococcal Protein A lacking the albumin-binding domain produced in E. coli
  • Bead size: 45-165μm
  • Bead Structure: 6% highly cross-linked agarose

 


Applications

  • Suitable for immunoaffinity chromatography and immunoprecipitation
Protocol
786-283
786-824
786-825
786-826
786-827
786-828
786-996
Material Safety Data Sheet
786-283
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786-824
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-825
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-826
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-827
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-828
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-996
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Antibody Handbook
Clean, Simple, Rapid Purification of Antibodies
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protein A, Protein G, Protein A/G and Protein L Binding Affinity A table highlighting the binding affinities of protein A, protein G, protein A/G and Protein L.  Designed to help in the choice of Well-Coated™ plates.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Protein Purification Handbook
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