Immobilized Protein A (11 Citations)

Immobilized Protein A is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). Protein A is coupled to agarose beads by reductive amination method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching (<5ng protein A/ml). Our immobilized protein A has a high binding capacity (>40mg/ml resin).

The Immobilized Protein A 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 0.2ml column is designed for 25-500µl of serum or other sample and can purify up to 6-8mg IgG, based on the 30-40mg/ml binding capacity of the resin.

The Immobilized Protein A 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.   Each 0.2ml column is designed for 0.5-2ml of serum or other sample and can purify up to 40mg IgG, based on the 30-40mg/ml binding capacity of the resin.

Available in 96-well spin plate formats for processing up to 96 samples. Other formats, including FPLC columns and magnetic beads.

Specific binding and elution buffers are also available.

Click here to request bulk or custom sizes.

Features

• High binding capacity: >40mg human IgG/ml resin
• Protein A Leaching: <10ng/mg IgG
• 30-300cm/hr flow velocity
• Ligand: Recombinant Staphylococcal Protein A lacking the albumin-binding domain produced in E. coli
• Bead size: 45-165μm
• Bead Structure: 6% highly cross-linked agarose

Applications

• Suitable for immunoaffinity chromatography and immunoprecipitation