10B Chemically Competent cells

G-Biosciences 10B Chemically Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. Chemically competent 10B E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Features:

• Clone large plasmids and BACs

• Reduced recombination of cloned DNA (recA1)

• Suitable for blue/white screening by α-complementation of the β-galactosidase gene

• Resistance to phage T1 (fhuA)

• Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)

Genotype:

Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)

Transformation efficiency:  1 x 10⁹ cfu/µg pUC19 DNA