OmniPrep™ for Tissue (4 Citations)
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A selection of genomic purification kits are offered that purify high quality genomic DNA from a wide variety of sources and for a wide array of applications.
Our OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications. These kits are fully scalable for large genomic DNA isolations. The procedure is slightly more involved to ensure ultra pure DNA.
Based on our popular OmniPrep™ system, the OmniPrep™ for Tissue kit has been modified to specifically isolate high quality genomic DNA from tissue samples, including fresh, frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from bodily fluids, including plasma, serum, amniotic fluid, semen and CSF. OmniPrep™ for Tissue also isolates high quality DNA from cultured cells, non-mammalian nucleated blood and gram-negative bacteria.
The kit isolates high purity (A260/A280 ratios of 1.8 to 2) DNA (about 100kbp) and the yield is 0.5 to 10μg/mg tissue, 25 to 50μg/ml gram negative bacteria culture and 0.1 to 40μg/ml body fluid, dependent on starting material and quantity.
If used according to protocol, the kit purifies DNA from a total of 2gm solid tissue, 1 x 109 cultured cells and 1 x 1010 gram-negative bacteria.
The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. Pure genomic DNA is isolated in 20 to 40 minutes, depending on the tissue type used.
OmniPrep™ for Tissue isolates genomic DNA from:
Mammalian tissue (fresh or frozen)
Ethanol or formalin fixed tissue
Paraffin embedded tissue
Bodily fluids, including plasma, serum, amniotic fluid, semen and CSF
Nucleated blood cells from bird, fish and frog
Gram negative bacteria
- High Yield: ~100kb genomic DNA
- Simple two tube method: 20-40 minutes
- No toxic chemicals, no phenol, no hazardous waste
- Extraction of pure genomic DNA from mammalian tissues
- Suitable for fresh, fixed, frozen or paraffin embedded tissue
|Material Safety Data Sheet|
|Molecular Biology Handbook||A guide to our products for DNA and RNA.|
- Beach, DG. et al (2020) Boronate techs. Pages: 125-128
- Lang, JR. et al (2020) Taxicology. doi.org/10.1016/j.tox.2020.152529
- Halbritter, DA et al (2019) Phylogeography and population genetics of pine butterflies: Sky islands increase genetic divergence. DA Halbritter Ecology and Evolution. DOI:10.1002/ece3.5793
- Mertens, J.A. et al (2006) Arch. Microbiol. 186:41