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Reducing Agent Compatible BCA Protein Assay

Reducing Agent Compatible Protein Assay
Catalog Description Size Price(USD) Qty
786-573
Bicinchoninic Acid (BCA) Protein Assay: Reducing Agent Compatible 250 Assays
$234.00
786-892
Bicinchoninic Acid (BCA) Protein Assay: Reducing Agent Compatible with Non Animal Protein Standard 250 Assays
$234.00

Reducing Agent Compatible BCA Protein Assay

Our Reducing Agent Compatible BCA Protein Assay is supplied with the Reducing Agent Compatibility Agent (RACA) that modifies reducing agents to limit their effect on the reduction of the assay’s copper ions, preventing inhibition of the assay.  The use of RACA allows for samples containing up to 5mM DTT, 10mM TCEP or 35mM ß-mercaptoethanol.The BCA Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.  The BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid (BCA) chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm that is linear for increasing protein concentrations between the range of 0.02-2mg/ml. The Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells. This kit is suitable for is for 250 x 1ml assays. The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

Features

  • Reducing agent compatible assay
  • Sensitive colorimetric assay
  • Linear range: 0.02-2mg/ml
  • Compatible with most ionic and non-ionic detergents

Applications

  • For measuring protein concentration in samples with reducing agents

For normal protein solutions, use the Bicinchoninic Acid (BCA) Protein Assays and for dilute protein samples, use the Micro Bicinchoninic Acid (BCA) Protein Assays.

Protocol
786-573
786-892
Material Safety Data Sheet
786-573
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786-892
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
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