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BCA Protein Assay for Dilute Samples

BCA Protein Assay for dilute protein samples
Catalog Description Size Price(USD) Qty
786-572
Micro Bicinchoninic Acid (BCA) Protein Assay 500 Assays/ 3,300 Micro-assays
$214.00
786-861
Assay Buffer (Micro BCA Reagent) 250ml
$55.00
786-862
Micro BCA Solution (BCA Reagent) 240ml
$99.00
786-895
Micro Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard 500 Assays/ 3,300 Micro-assays
$214.00

BCA Protein Assay for Dilute Samples

The Micro BCA Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the BCA Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562nm. The assay uses concentrated solutions and extended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations. The Micro BCA Protein Assay is suitable for quantifying protein solutions in 500 x 1ml assays or in >3,300 micro-well assays. The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

Features

  • Sensitive, colorimetric assay for dilute protein samples
  • Linear range: 0.5-20µg/ml
  • Compatible with most non-ionic and ionic detergents

Applications

  • For dilute protein samples (0.5-20µg/ml)

For information on our regular Bicinchoninic Acid (BCA) Protein Assay, click here.  For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.

Protocol
786-572
786-861
786-862
786-895
Material Safety Data Sheet
786-572
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786-861
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-862
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-895
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
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