-500x500.jpg "Immobilized Iminobiotin Resin")
| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-599 | Immobilized Iminobiotin | 5ml resin | $230.00 |
Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.
The normal biotin-avidin complex requires strong denaturing buffers, i.e. 8M guanidine•HCl, to denature the avidin and release the biotin, which obviously destroys the native and functional aspects of the avidin. The iminobiotin-avidin complex will form at >pH9.5 and can be dissociated at pH4.0 with gentle elution buffers, including 50mM ammonium acetate, pH4.0 with 0.5M NaCl.
Click here to request bulk or custom sizes.
Features
Applications
Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.
The normal biotin-avidin complex requires strong denaturing buffers, i.e. 8M guanidine•HCl, to denature the avidin and release the biotin, which obviously destroys the native and functional aspects of the avidin. The iminobiotin-avidin complex will form at >pH9.5 and can be dissociated at pH4.0 with gentle elution buffers, including 50mM ammonium acetate, pH4.0 with 0.5M NaCl.
Click here to request bulk or custom sizes.
Features
Applications
