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Labeling & Conjugation

G-Biosciences offer a vast array of products for protein labeling and conjugations. Both fluorescent and biotin labeling kits are available as well as products for agarose conjugation and accessories. 

Fluorescent dyes have become a common tool in laboratories for the visualization and studying of proteins, whether they are attached directly to the protein of interest or to antibodies specific for the protein of interest. 

G-Biosciences researchers have developed two kits for the rapid and efficient labeling of proteins, including antibodies, with a fluorescent green (FITC) or red ((5/6) TAMRA-SE) dye.  Each kit is supplied with five of our single-use OneQuant™ fluorescent reagents that protect the dyes from exposure to light and minimize hands-on time.  The following evaluates labeling of two different proteins with fluorescent dyes.

 

HOOK-Biotin kits come with all the necessary reagents and equipment for optimization of reaction conditions, efficient labeling, removal of unbound biotin and quantification of biotin labeling.  In addition to highly efficient labeling, the HOOK-Biotin kits offer the advantage of being supplied with micro dialysis units and a specific Optimizer Buffer.  These greatly simplify the labeling process and ensure high levels of biotin labeling.

 

Download the Protein Labeling & Conjugation Handbook

HOOK™-NHS-LC-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abunda..
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HOOK™ NHS-LC-LC-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abu..
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HOOK™ NHS-SS-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abunda..
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HOOK™ PEG2-Iodoacetyl-Biotin reacts with protein free sulhydryl groups.   Sulfhydryl reactive reagents are more specific and react only w..
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HOOK™ PFP-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abundant ..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research..
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HOOK™ Sulfo-NHS-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abu..
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HOOK™-Sulfo-NHS-LC-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most ..
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HOOK™ Sulfo-NHS-LC-LC-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the mo..
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HOOK™ Sulfo-NHS-SS-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most ..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is ra..
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Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allo..
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Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Bi..
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Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be per..
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The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of th..
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The HOOK™ HRP PLUS labeling kit is a high efficiency horseradish peroxidase (HRP) enzyme labeling kit for tagging the enzyme with proteins. ..
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