| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-1811 | Immobilized β-cyclodextrin | 10ml resin | $616.00 | |
| 786-1812 | Immobilized β-cyclodextrin | 100 ml resin | $2,813.00 |
Immobilized β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism. The misfolded proteins or inclusion bodies are solubilized with detergent such as Triton X-100 in the first step to avoid protein aggregation. In the following step the detergent treated protein is passed through immobilized β-cyclodextrin agarose preequilibrated with refolding buffer. Cyclodextrin strips away the detergent allowing proper refolding of proteins.
Features:
Application(s):
Immobilized β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells
Immobilized β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism. The misfolded proteins or inclusion bodies are solubilized with detergent such as Triton X-100 in the first step to avoid protein aggregation. In the following step the detergent treated protein is passed through immobilized β-cyclodextrin agarose preequilibrated with refolding buffer. Cyclodextrin strips away the detergent allowing proper refolding of proteins.
Features:
Application(s):
Immobilized β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells
