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Affinity Purification Resins and Methods

 

An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more.  Efficient Avidin-Streptavidin and Neutravidin purification is simple and affordable with our immobilized biotin and iminobiotin resins. High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin. Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. A more economical substitute for Immobilized p-Aminophenyl Phosphoryl Choline, Immobilized O-Phosphorylethanolamine is also available for the purification of C-reactive protein.  A human recomboninant C-reactive protein is also offered. Purify your GST-tagged or His-tagged proteins with GST-tagged or His-tagged protein purification resins or kits.  Isolate proteins of the hemostatic pathways, growth hormones and growth factors, lipoproteins and other physiological markers with immobilized heparin and lectins.  Rapidly purify lectins, galactose-binding molecules, agglutinins, N-acetylgalactosamine-binding, N-acetylglucasomine-binding and carbohydrate binding molecules with our lectin purification reagents. A variety of non-porous, high antibody binding capacity, cost effective magnetic beads are available. Immobilized boronic acid is designed specifically for the simple isolation of small molecular weight compounds that have cis-diol groups such as ribonucleotide and oligonucleotide RNA. 

 
Download the Protein Purification Handbook for a full list of related products
Download the Purification Resins Handbook for Complete Product List 
Gene/Protein:
Immobilized Folic Acid resin is designed for affinity purification of folate binding proteins. Features: Matrix: 6% cross-linked agarose Matrix acivation: epoxy Extent of labeling: 4-7 µmol folate/ml resin Storage buffer: 20% ethanol Applications: Immobilized Fol..
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Gene/Protein:
Immobilized Guanosine 5′-triphosphate is affinity resin used in purifying guanosine binding proteins   Click here to request bulk or custom sizes. Features: GTP coupled to 6% cross-linked Agarose Extent of labeling is 1- 5 µmol GTP per ml resin  ​Shipped as h..
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Gene/Protein:
Immobilized Human IgG is affinity resin used to purify Protein A and Protein A tagged recombinant proteins and is suitable for tandem affinity purification (TAP) of protein complexes. Click here to request bulk or custom sizes. Features: Matrix: 6% cross-linked agarose Ligand: Human Ig..
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Gene/Protein:
Immobilized L-Tartrate is designed for affinity purification of L-Tartrate binding proteins such as human prostatic acid phosphatase. Features: Matrix: 6% cross-linked Agarose ​Coupling is covalent through linkage of hydroxyl group of  tartrate to the pre-activated agarose. Extent ..
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Gene/Protein:
Immobilized L-Thyroxine is designed for affinity purification of L-Thyroxine binding proteins. Features: ​Matrix: 6% cross-linked agarose Extent of labeling: 3-8 μmol per ml resin Storage buffer: 20% ethanol   Application(s): Immobilized L-Thyroxine&n..
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Gene/Protein:
Immobilized Methotrexate is designed for affinity purification of enzyme dihydrofolate reductase (DHFR). DHFR has been purified from a variety of sources by conventional protein purification procedures. However due to small amount of DHFR activity present in cells and tissues, the conventional metho..
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Gene/Protein:
Mouse IgG Agarose is suitable for immunoprecipitation and Immunoaffinity purifications.    Click here to request bulk or custom sizes.   Features: Matrix: 6% cross-linked agarose Ligand: Mouse IgG Extent of labeling: 0.5-1mg of Mouse IgG per ml resin. Storage b..
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Gene/Protein:
Immobilized N6-(4-Aminobenzyl)Adenosine is made by  covalent coupling of N6-(4-Aminobenzyl)Adenosine with 6% cross-linked agarose beads.  Immobilized N6-(4-Aminobenzyl)Adenosine is used in purification of A2-adenosine receptors.   Click here to request bulk or custom sizes. F..
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G-Biosciences Immobilized O-Phosphorylethanolamine is used in purification of calcium binding C-reactive proteins such as canine CRP and human CRP. For details of Purification of C-Reactive proteins on G-Biosciences Immobilized O-Phosphorylethanolamine Resin, download the application note. &nbs..
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G-Biosciences' Immobilized p-Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids.   CRP, C-reactive protein, is a pentameric protein&..
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Gene/Protein:
Immobilized PEG 20,000 is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. Immobilized PEG 20,000 is a hydrophobic interaction media which is considered more efficient in enhancing refolding than o..
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Gene/Protein:
Immobilized Phosphatidyl-L-Serine is made by covalent binding of Phosphatidyl-L-Serine with 6% cross-linked agarose beads. Immobilized Phosphatidyl-L-Serine is used in protein pull-down experiments to identify and characterize lipid binding proteins. It is also used in purification of lipid bindi..
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