G-Biosciences' Protein A Magnetic Beads are Fe3O4 beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein A is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments.
The use of magnetic beads offers several distinct advantages of traditional immunoprecipitation experiments include a significant reduction in time and non-specific protein binding.
A simple protocol involves the addition of your antibody of choice to the beads, which bind the Fc region (See Table 1, figure 1) during a short incubation. The tube is placed on a magnet and the supernatant is removed by aspiration. The antibody bound magnetic beads can be used in a variety of downstream processes including:
- Immunoprecipitations (Figure 2)
- Co-immunoprecipitations (Figure 4)
- Chromatin immunoprecipitation (ChIP)
- Small-scale IgG Purification and antibody labeling (Figure 3)
- Protein Isolation
Features
- 1µm beads
- 260µg human IgG/ml
- Significant reduction in background due to non-specific binding
- Simple, short procedure
- No costly columns required
- No time consuming centrifugations required
- Gentle separation preserves native protein interactions
Applications
- Immunoprecipitations
- Co-immunoprecipitations
- Chromatin immunoprecipitation (ChIP)
- Small-scale IgG Purification and antibody labeling
- Protein Isolation
