Magnetic bead purification techniques offer some significant advantages over traditional protein and nucleic acid purification techniques. Rapid purification of proteins and antibodies is simple with coated magnetic beads. Save time and money by substituting your column based nucleic acid purification with a silica coated magnetic bead technique.
Protein A and Protein G Magnetic Beads are a simple and affordable way to purify your antibodies of choice. Recombinant Protein A or Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments.
Molecules can be coupled to the free amine by numerous amine-reactive methods utilizing Carboxyl Magnetic Beads. The magnetic bead consists of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the carboxyl groups (-COOH) are joined to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the magnetic beads excellent dispersion ability and easy handling property in a wide variety of buffers.
The Amine Magnetic Beads with surface-reactive amino groups allow immobilization of ligands such as proteins, peptides, carbohydrates or other target specific molecules. Immobilization of ligands can be through reductive amination of aldehyde or ketones without prior activation of the bead surface. Alternatively, carbodiimide cross-linkers can be used to couple ligands to the amines via their carboxyl groups. Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligands.
G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged nickel covalently bound to the surface dextran of the beads. Due to the high affinity, Ni-NTA Magnetic Beads can be used for capturing 6X His-tagged proteins.
Efficient and simple nucleic acid binding is possible with G-Biosciences’ Silica Magnetic Beads. Purify plasmid DNA, genomic DNA, RNA, or PCR products in less time and for less cost than column purification.
For efficient pull down of magnetic beads in 1.5mL and 2mL tubes we offer a Magnetic Stand. This stand contains a powerful magnet that pulls beads to the side of the tube and allows removal of supernatant with no bead contamination.