G-Trap™ G-Acryl Desalting Columns
G-Trap™ G-Acryl Desalting columns are ready to use prepacked columns for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography. G-Trap™ G-Acryl Desalting columns are mostly used for removal of salt or buffer exchange before or after different chromatographic steps. G-Trap™ G-Acryl Desalting columns can be used for separation of proteins based on their sizes.
G- Biosciences offer three types of desalting columns based on different exclusion limit, namely G-Trap™ G-Acryl 600 Desalting Column, G-Trap™ G-Acryl 100 Desalting Column and G-Trap™ G-Acryl 1200 Desalting Column. The characteristics of G-Trap™ G-Acryl Desalting column resin are shown below in table 2
The G-Trap™ G-Acryl columns consist of porous polyacrylamide beads that are extremely hydrophilic and essentially free of charge. The resin is compatible with the following reagents:
- Dilute Organic Acids
- Urea [8M]
- Guanidine-HCl [6M]
- Chaotropic Agents
- Reducing Agents (DTT, 2-mercaptoethanol)
- Detergents (SDS, CHAPS, Triton® X-100, etc)
- Alcohols (<20%)
- Formamide
The G-Trap™ columns are made of biocompatible polypropylene, which does not interact with biomolecules. The column has a stopper at the inlet and snap-off end at the outlet. The characteristics of the column are listed in Table1.
Table 1: G-Trap™ G-Acryl Desalting Column
Features |
1 ml column |
5 ml column |
Column Volume |
1 ml |
5 ml |
Column Dimensions |
0.7 x 2.5 cm |
1.6 x 2.5 cm |
Column Hardware Pressure Limit |
0.5 MPa |
0.5 MPa |
Column hardware |
Polypropylene |
Polypropylene |
NOTE: The pressure over the packed volume varies depending upon the type of medium or matrix, sample or liquid viscosity, and the column tubing used.
Table 2: G-Trap™ G-Acryl Desalting Column resin
Features |
G-Trap™ G-Acryl 600 Desalting Column |
G-Trap™ G-Acryl 100 Desalting Column |
G-Trap™ G-Acryl 1200 Desalting Column |
Bead size |
90-180 µm |
45-90 µm |
90-180 µm |
Void volume |
~0.3 ml for 1 ml column and ~1.5 ml for 5 ml column |
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Sample Load volume |
0.03 ml- 0.3 ml for 1 ml column and 0.75-1.5 ml for 5 ml column |
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Exclusion limit (Mr) |
6,000 |
1,800 |
20,000 |
Maximum Pressure |
1 bar [0.1 MPa] (15 psi) |
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Recommended Flow rate |
15-20 cm/hr |
5-10 cm/hr |
15-20 cm/hr |
Chemical stability |
All commonly used buffers, 8M urea, 6M guanidine hydrochloride and all non-ionic detergents. Lower alcohols such as methanol, ethanol and propanol can be added to buffers at <25% V/V |
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pH stability |
2 to 10 |
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Storage |
20 % ethanol at 4°C to 30°C |