Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds.
This trypsin is purified from bovine pancreas, 1X crystallized, dialyzed against 1mM HCl and lyophilized.
>150 TAME untis/ mg protein. One Unit hydrolyzes 1µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10mM calcium.
For mass spectrometry sequence analysis, we recommend our ultra-pure MSG-Trypsin™ that is specifically designed for mass spectrometry.
- Synonym: Cocoonase
- CAS Number: 9002-07-7
- Linear Formula: C6H15O12P3
- Molecular Weight: 372.1
- EC Number: 126.96.36.199
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|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|