Epoxy-Activated Agarose (Dry Form)

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.

Epoxide-Acivated Agarose is prepared by immobilization of oxiranes such as 1,4-butanediol diglycidyl ether onto matrix. This pre-activated resin provide hydrophilic 12 atom spacer arm which enables binding of small ligand molecules such as amino acids, monosaccharide, peptides and carbohydrates. 

Epoxy-Activated Agarose  find widespread application in affinity chromatography involving purification of antigen, antibody, lectin, glycoproteins  etc.

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Features:

• Resin Active group: epoxy
• Resin Active group density: 20-40 µM/ ml hydrated resin.
• Group to be coupled: -NH₂, -OH or -SH
• 45-165µm particle size range
• Spherical, highly cross-linked 6% agarose
• Resin spacer arm: 12 atom hydrophilic spacer arm
• Coupling conditions: pH 7.5-8.5 for thiol ligands, pH 9-10 for amine ligands and pH 11-13 for hydroxyl ligands at 4 to 37°C depending upon ligand stability for 16 hrs
• pH stability: 2-13, ligand dependent
• 1 gm of dry resin swells to 5-6 ml.
• Storage: 2- 8°C, desiccated

Application(s):

• Epoxy-Activated Agarose is preactivated resin used for affinity chromatography.
• It can couple various small and big molecule ligands via their amine, thiol or hydroxyl groups
•  Epoxy-Activated Agarose  find widespread application in affinity chromatography involving purification of antigen, antibody, lectin, glycoproteins  etc