OmniPrep™ for Tissue
Based on our popular OmniPrep™ system, the OmniPrep™ for Tissue kit has been modified to specifically isolate high quality genomic DNA from tissue samples, including fresh, frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from bodily fluids, including plasma, serum, amniotic fluid, semen and CSF. OmniPrep™ for Tissue also isolates high quality DNA from cultured cells, non-mammalian nucleated blood and gram-negative bacteria.
The kit isolates high purity (A
260/A
280 ratios of 1.8 to 2) DNA (~100kbp) and the yield is 0.5-10μg/mg tissue, 25-50μg/ml gram negative bacteria culture and 0.1-40μg/ml body fluid, dependent on starting material and quantity.
If used according to the protocols this kit purifies DNA from a total of 2gm solid tissue, 1x10
9 cultured cells and 1x10
10 gram- negative bacteria.
The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. Pure genomic DNA is isolated in 20-40 minutes, depending on the tissue type used. OmniPrep™ for Tissue isolates genomic DNA from:
- Mammalian tissue (fresh or frozen)
- Cultured cells
- Ethanol or formalin fixed tissue
- Paraffin embedded tissue
- Bodily fluids, including plasma, serum, amniotic fluid, semen and CSF
- Nucleated blood cells from bird, fish and frog
- Gram negative bacteria
FEATURES
- High Yield, ~100kb genomic DNA.
- A260/A280 between 1.8 and 2.0.
- Simple two tube method, 20-40 minutes.
- No toxic chemicals, no phenol, no hazardous waste.
APPLICATIONS
- Extraction of pure genomic DNA from mammalian tissues.
- Suitable for fresh, fixed, frozen or paraffin embedded tissue.
- Extraction of high quality, 100kb genomic DNA.